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KMID : 0613820120220121672
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Journal of Life Science
2012 Volume.22 No. 12 p.1672 ~ p.1679
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Effects of High Stocking Density on the Expressions of Stress and Lipid Metabolism Associated Genes in the Liver of Chicken
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An Young-Sook
Jang In-Surk
Park Jeong-Geun
Sohn Sea-Hwan
Moon Yang-Soo
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Abstract
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The effect of high stocking density (HSD) on the expression of stress and lipid metabolism associated genes in the liver of broiler chickens was examined by chicken genome array analysis. The chickens in a control group were randomly assigned to a 495㎠/bird stocking density, whereas the chickens in a HSD group were arranged in a 245㎠/bird stocking density with feeding ad libitum for 35 days. The chickens assigned to the HSD group had a significantly lower body weight, weight gain, and feed intake compared with those of the control group (p<0.05). The mortality of chickens was higher in the HSD group than in the control group. The microarray analysis indicated up-regulation of stress associated genes such as HMGCR, HSP90α, HSPA5 (GRP78/Bip), DNAJC3 and ATF4, and down-regulation of interferon-r and PDCD4 genes. The endoplasmic reticulum stress associated genes, HSPA5 (GRP78/Bip), DNAJC3 and ATF4, were highly expressed in the HSD group. The genes, ACSL5, TMEM195 and ELOVL6, involved in fatty acid synthesis, were elevated in the HSD group. The genes, ACAA1, ACOX1, EHHADH, LOC423347 and CPT1A, related to fatty acid oxidation, were also activated in the HSD group. These results suggest that a HSD rearing system stimulates the genes associated with fatty acid synthesis as well as fatty acid oxidation in the liver of broiler chickens.
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KEYWORD
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Chicken, high stocking density, stress, microarray, gene expression
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